experiment id title description array_ID author contact name contact email contact institute publication 1 Differential modulation of photosynthesis, signaling, and transcriptional regulation between tolerant and sensitive tomato genotypes under cold stress The
wild
species
Solanum
habrochaites
is
more
cold-tolerant
than
the
cultivated
tomato
(S.
lycopersicum).
To
explore
the
mechanisms
responsible
for
cold
tolerance
in
S.
habrochaites,
one
cold-tolerant
line
(LA3969)
was
identified
from
S.
habrochaites
LA1777
introgression
lines
(ILs).
The
differences
in
physiology
and
global
gene
expression
between
cold-tolerant
(LA1777
and
LA3969)
and
-sensitive
(LA4024)
genotypes
under
cold
stress
were
further
investigated.
The
two
tolerant
genotypes
exhibited
less
severe
membrane
damage,
higher
peroxidase
activity,
lower
ascorbate
peroxidase
activity,
and
a
slightly
higher
reactive
oxygen
species
(ROS)
accumulation.
Transcriptome
analysis
identified
296
genes
differentially
expressed
between
tolerant
and
sensitive
genotypes
under
cold
stress.
More
transcripts
categorized
into
‘response
to
stress’,
‘biosynthetic
process’,
‘signal
transduction’,
and
‘transcription’,
were
up-regulated
in
the
two
tolerant
genotypes.
However,
more
biological
processes
were
significantly
repressed
in
LA4024,
such
as
‘auxin
homeostasis’,
‘oxidation
reduction’,
‘calcium
ion
homeostasis’,
and
‘regulation
of
hormone
levels’.
The
two
tolerant
genotypes
showed
less
repression
of
many
photosynthesis-related
genes,
and
maintained
higher
PSII
activities
than
the
sensitive
one.
Many
transcripts
involved
in
ROS
homeostasis,
hormone
metabolism
and
signaling
pathways,
and
calcium
regulation
were
modulated
between
tolerant
and
sensitive
genotypes.
ROS,
calcium,
and
hormones
as
signaling
molecules
may
play
critical
roles
in
regulating
gene
expression
in
response
to
cold
stress.
Moreover,
the
expression
of
various
transcription
factors,
post-translational
proteins,
and
metabolic
enzymes
were
also
specifically
modulated.
These
special
modifications
may
play
important
roles
in
conferring
cold
tolerance
in
tomato.
These
results
not
only
provide
new
insights
into
the
molecular
mechanisms
of
cold
tolerance
in
tomato,
but
also
provide
potential
candidate
genes
for
genetic
improvement. A01 Hui Liu Zhibiao Ye zbye@mail.hzau.edu.cn Key
Laboratory
of
Horticultural
Plant
Biology,
Ministry
of
Education,
Huazhong
Agricultural
University,
Wuhan,
China