Domain-wise effect of AvrPto on the tomato transcriptome 6 hours post inoculation

Experiment description: A P. syringae mutant deleted in all well-expressed effector proteins delivered AvrPto expressed from a gene in trans on a plasmid. AvrPto forms are WT, inactivated in either the CD-loop or C-terminal domains, or with both domains inactivated. 6 hours after vacuum infiltration at OD 600 0.02 with 0.02% silwet and 10 mM MgCl2, the samples were collected in tubes, then flash frozen in liquid nitrogen for subsequent analysis.

Author: Jay Worley

Contact: Greg Martin (, Boyce Thompson Institute, Cornell University