Impact of sucrose retrieval upon ex vitro acclimatization
Hybridization: vitro_Suc+24h/vitro_Suc-24h leaf |
| Hybridization protocol |
| Hybridization solution | N/A |
|---|
| Solution concentration | N/A |
|---|
| Blocking agent | N/A |
|---|
| Wash procedure | Arrays were washed twice in both low and high stringency buffers as suggested by the SlideHyb buffer #1 protocol (Applied Biosystems). |
|---|
| Labeled Target Quantity | 20 ug |
|---|
| Hybridization Time | 17 hr |
|---|
| Hybridization Volume | N/A |
|---|
| Hybridization Temperature | 42oC |
|---|
| Hybridization Instrument | SlideBooster instrument |
|---|
|
| Scan information |
| Scan hardware | VersArray ChipReader |
|---|
| scan software | ChipReader 3.1 |
|---|
|
| Image analysis information |
| Software | Array-Pro Analyzer v.4.5 software |
|---|
| Analysis algorithm | N/A |
|---|
|
| Normalization information |
| Normalization software | Microarray Analysis of Variance (MAANOVA) software |
|---|
| Normalization algorithm | global Loess method |
|---|
| Normalization strategy | N/A |
|---|
|
