Tomato root culture and Al treatment

Hybridization: Al-treated/control

Hybridization protocol
Hybridization solutionN/A
Solution concentrationN/A
Blocking agentN/A
Wash procedureN/A
Labeled Target Quantity1.5 ug (~ 100 pmol) of Cy3 and 1.5 ug Cy5
Hybridization TimeN/A
Hybridization VolumeN/A
Hybridization TemperatureN/A
Hybridization InstrumentN/A

Scan information
Scan hardwareGenePix 4000B slide scanner
scan software

Image analysis information
SoftwareGenepix 6.0 software
Analysis algorithmN/A

Normalization information
Normalization softwaremarray package under R
Normalization algorithmPrint-tip specific Lowess Normalization
Normalization strategyTotal Array