Tomato root culture and Al treatment
Hybridization: Al-treated/control |
| Hybridization protocol |
| Hybridization solution | N/A |
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| Solution concentration | N/A |
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| Blocking agent | N/A |
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| Wash procedure | N/A |
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| Labeled Target Quantity | 1.5 ug (~ 100 pmol) of Cy3 and 1.5 ug Cy5 |
|---|
| Hybridization Time | N/A |
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| Hybridization Volume | N/A |
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| Hybridization Temperature | N/A |
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| Hybridization Instrument | N/A |
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|
| Scan information |
| Scan hardware | GenePix 4000B slide scanner |
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| scan software | |
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|
| Image analysis information |
| Software | Genepix 6.0 software |
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| Analysis algorithm | N/A |
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|
| Normalization information |
| Normalization software | marray package under R |
|---|
| Normalization algorithm | Print-tip specific Lowess Normalization |
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| Normalization strategy | Total Array |
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