Transcription profiling of tomato strains during fruit set

Hybridization: AS-IAA9/WT (4 day Post-anthesis)

Hybridization protocol
Hybridization solutionN/A
Solution concentrationN/A
Blocking agentN/A
Wash procedurewashed twice at 42oC (90 sec and 5 min respectively) in 2x SSC/0.1% SDS, then twice for 2 min in 1x SSC at ambient temperature, and twice for 1 min in 0.1x SSC at ambient temperature.
Labeled Target QuantityStarting material 5µg of total RNA
Hybridization Time14-16h
Hybridization VolumeN/A
Hybridization Temperature42oC
Hybridization InstrumentN/A

Scan information
Scan hardwareGenePix Personal 4100A
scan softwareGenePix Pro [Axon Instruments]

Image analysis information
SoftwareGenepix Pro 3 software
Analysis algorithmN/A

Normalization information
Normalization softwaremarray package under R
Normalization algorithmPrint-tip specific Lowess Normalization
Normalization strategyTotal Array