Tomato molecular resource order information

This site has been developed to facilitate distribution of tomato cDNA and BAC clones developed with support from the NSF Plant Genome Program and as part of the International SOL project focused on tomato genome sequencing. As we are not supported for resource distribution and requests are extensive this is being done on a cost recovery basis. Additional information regarding tomato ESTs, fosmid and BAC clones and libraries can be found at the Solanaceae Genomics Network (SGN).

A number of important points should be kept in mind when ordering tomato cDNA or BAC clones. As is typical for all large scale clone programs there are errors in annotation. Due to a combination of human and machine errors, the clone IDs found in SGN, TIGR, GenBank or other databases may not always match the indicated sequences. As a result, if you order clone X and sequence it you may find it is not what you wanted. For example, in the tomato EST collection we estimate an average error rate of approximately 10% (though this can be significantly higher in specific libraries or segments of libraries). You should account for this error in your clone requests and this site helps you to do so in several ways. First, a subset of clones including those used in the microarray have been re-sequenced and are thus the most accurate (though still not 100%). Our clone search tool will assist you in identifying clones from this subset that match your sequence of interest (You can blast against the whole microarray re-sequenced collection from here or against all available full BAC, BAC end or fosmid end sequences at SGN). We also strongly suggest that you request additional clones from the EST contig if available (you can go to SGN to get additional member information) and especially if re-sequenced clones are not available.

You should verify any clones you receive via sequencing (to verify BAC or fosmid end sequence or to verify cDNA insert sequence). Clones that are not as expected cannot be replaced for free unless error on our part is apparent.