Tomato root culture and Al treatment
Hybridization: Al-treated/control
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Hybridization protocol
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| Hybridization solution | N/A |
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| Solution concentration | N/A |
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| Blocking agent | N/A |
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| Wash procedure | N/A |
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| Labeled Target Quantity | 1.5 ug (~ 100 pmol) of Cy3 and 1.5 ug Cy5 |
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| Hybridization Time | N/A |
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| Hybridization Volume | N/A |
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| Hybridization Temperature | N/A |
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| Hybridization Instrument | N/A |
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Scan information
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| Scan hardware | GenePix 4000B slide scanner |
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| Scan software | |
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Image analysis information
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| Software | Genepix 6.0 software |
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| Analysis algorithm | N/A |
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Normalization information
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| Normalization software | marray package under R |
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| Normalization algorithm | Print-tip specific Lowess Normalization |
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| Normalization strategy | Total Array |
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