Transcriptional Profiling of high pigment-2dg Tomato Mutant Fruit
Hybridization: Manapal/WT (red)
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Hybridization protocol
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| Hybridization solution | N/A |
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| Solution concentration | N/A |
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| Blocking agent | N/A |
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| Wash procedure | Microarray slides were washed seven times for 10 min each: at room temperature in 2XSSC/0.1% SDS- twice, room temperature in 0.2XSSC/0.1% SDS- twice, room temperature with 0.2XSSC- twice, and once in 0.2XSSC at 42oC. |
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| Labeled Target Quantity | N/A |
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| Hybridization Time | 14 to 16 h |
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| Hybridization Volume | N/A |
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| Hybridization Temperature | 42<sup>o</sup>C |
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| Hybridization Instrument | covered hybridization oven |
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Scan information
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| Scan hardware | GenePix Personal 4100A |
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| Scan software | GenePix Pro [Axon Instruments] |
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Image analysis information
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| Software | Genespring 7.2 software |
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| Analysis algorithm | N/A |
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Normalization information
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| Normalization software | marray package under R |
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| Normalization algorithm | Print-tip specific Lowess Normalization |
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| Normalization strategy | Total Array |
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