Transcription profiling of tomato strains during fruit set
Hybridization: AS-IAA9/WT (beginning of stage)
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Hybridization protocol
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| Hybridization solution | N/A |
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| Solution concentration | N/A |
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| Blocking agent | N/A |
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| Wash procedure | washed twice at 42oC (90 sec and 5 min respectively) in 2x SSC/0.1% SDS, then twice for 2 min in 1x SSC at ambient temperature, and twice for 1 min in 0.1x SSC at ambient temperature. |
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| Labeled Target Quantity | Starting material 5µg of total RNA |
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| Hybridization Time | 14-16h |
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| Hybridization Volume | N/A |
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| Hybridization Temperature | 42<sup>o</sup>C |
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| Hybridization Instrument | N/A |
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Scan information
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| Scan hardware | GenePix Personal 4100A |
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| Scan software | GenePix Pro [Axon Instruments] |
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Image analysis information
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| Software | Genepix Pro 3 software |
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| Analysis algorithm | N/A |
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Normalization information
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| Normalization software | marray package under R |
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| Normalization algorithm | Print-tip specific Lowess Normalization |
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| Normalization strategy | Total Array |
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