Transcription profiling of tomato plants at three time points in the light:dark cycle
Hybridization: ZT8/ZT0
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Hybridization protocol
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| Hybridization solution | |
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| Solution concentration | |
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| Blocking agent | |
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| Wash procedure | washed in decreasing SSC concentrations and 0.1% SDS at 55 oC and room temperature, respectively. The last wash was carried out in 0.1x SSC at room temperature. |
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| Labeled Target Quantity | 200 pmoles of purified Cy3- and Cy5-labelled aRNAs |
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| Hybridization Time | overnight |
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| Hybridization Volume | |
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| Hybridization Temperature | 55<sup>o</sup>C |
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| Hybridization Instrument | automatic hybridization station |
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Scan information
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| Scan hardware | ScanArray Lite (Perkin-Elmer) |
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| Scan software | N/A |
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Image analysis information
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| Software | ScanArray Express (Perkin-Elmer) |
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| Analysis algorithm | N/A |
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Normalization information
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| Normalization software | Excel Spreadsheets used to calculate total signal |
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| Normalization algorithm | N/A |
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| Normalization strategy | Total array |
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