Differential modulation of photosynthesis, signaling, and transcriptional regulation between tolerant and sensitive tomato genotypes under cold stress
Hybridization: LA3969 cold stress/LA3969 no stress
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Hybridization protocol
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| Hybridization solution | N/A |
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| Solution concentration | 3X SSC, 0.2%SDS, 5X Denhart's, 25%DMF |
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| Blocking agent | N/A |
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| Wash procedure | The arrays were washed with 0.2% SDS, 2X SSC at 42oC for 5 min, followed by washing with 0.2% SSC for 5 min at room temperature |
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| Labeled Target Quantity | 5ug cRNA |
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| Hybridization Time | Overnight |
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| Hybridization Volume | 80 ul |
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| Hybridization Temperature | 42<sup>o</sup>C |
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| Hybridization Instrument | Corning microarray hybridization chamber in 42<sup>o</sup>C incubator |
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Scan information
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| Scan hardware | LuxScan 10KA confocal laser scanner |
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| Scan software | N/A |
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Image analysis information
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| Software | LuxScan 3.0 |
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| Analysis algorithm | N/A |
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Normalization information
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| Normalization software | marray package under R |
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| Normalization algorithm | Print-tip specific Lowess Normalization |
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| Normalization strategy | Total Array |
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